Edmund Optics® 120i Plan APO Infinity Corrected Objectives

TECHSPEC^® 120i Plan APO Infinity Corrected Objectives are infinite conjugate microscope objectives designed to reduce the overall weight and size of an imaging system while maintaining optical performance. These objectives allow for a reduction in overall system length, by eliminating the requirement for the objectives to be parfocal to each other. TECHSPEC^® 120i Plan APO Infinity Corrected Objectives feature c-mount threading to provide easy integration into a wide range of machine vision and life science systems. These objectives are ideal for biomedical and machine vision applications such as microscopy, flow cytometry, pharmaceutical inspection, and assembly line or fault inspection.

Zea Seed, Brightfield, 120i 10X Plan APO Infinity Corrected Objective

Kidney, Brightfield, 120i 5X Plan APO Infinity Corrected Objective

Lilium Ovary, Brightfield, 120i 10X Plan APO Infinity Corrected Objective

Semiconductor Wafer, In-Line, 120i 20X Plan APO Infinity Corrected Objective

Semiconductor Wafer, In-Line, 120i 10X Plan APO Infinity Corrected Objective

Semiconductor Wafer, Darkfield, 120i 5X Plan APO Infinity Corrected Objective

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Basic Digital Microscope System

C-Mount Camera

#18-504 Camera Tube

C-Mount M29 x 0.75

#18-505 Tube Lens

M29 x 0.25 Dovetail

Dovetail

#18-506
Objective to Tube Lens Spacer

C-Mount

120i Plan APO Infinity
Corrected Objectives

Figure 1: Basic Microscope System with TECHSPEC^® 120i Plan APO Infinity Corrected Objectives

The minimum required components for a basic digital microscope system are a TECHSPEC^® 120i Plan APO Infinity Corrected Objective, camera tube, tube lens, lens tube spacer, and an industrial C-Mount camera (Figure 1). Learn more about assembling a basic system in either a vertical or horizontal configuration here.

Fluorescence Microscope System

C-Mount Camera

#18-504 Camera Tube

C-Mount M29 x 0.75

#18-505 Tube Lens

M29 x 0.25 Dovetail

Dovetail

#18-508 Filter Cube * *Designed for use with Fluoresence Fiber Kits One Required

C-Mount

120i Plan APO Infinity
Corrected Objectives

Figure 2: Fluorescence Microscopy System with TECHSPEC^® 120i Plan APO Infinity Corrected Objectives

For life science systems requiring fluorescence imaging, TECHSPEC^® Fluorescence Filter Sets can also be incorporated into the system with the available accessories. Learn more about assembling these fluorescence configurations here.

Featured Products

120i Plan APO Infinity Corrected Objectives

• 5X, 10X, 20X magnifications available
• Designed for use with 120mm tube lens

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120i Tube Lens and Accessories

• 120mm tube lens for use with C-mount camera
• Additional accessories for filters and mounting

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Fluorescence Filter Sets

• Excitation, emission, and dichroic filters
• >93% transmission and OD 6 blocking

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Numerical aperture (NA) is an important specification to define for a microscope objective because it describes the objective’s ability to gather light at a fixed working distance. The NA is often assumed to describe an objective’s resolving power; however, this is not an accurate assumption. The equation below is the first-order equation used to calculate NA. This equation is useful for understanding NA on-axis, but it does not consider field changes for off-axis performance. In addition, this equation does not consider tolerances during the manufacturing and assembly of microscope objectives that can impact the performance.

Figure 1 below demonstrates the off-axis performance of the 5X 120i Plan APO Infinity Corrected Objective compared to another 5X Plan APO infinity corrected objective from another manufacturer that has the same NA of 0.14. Performance off-axis is better with the 120i Objective in the corner of a 1.1” sensor, indicating that NA is not the defining factor for the performance of an objective.

Figure 4. Contrast Comparison using a USAF Resolution Test Target on a 1.1” Sensor

a) Image with 5X 120i Plan APO Infinity Corrected Objective showing 20% contrast at 330 lp/mm

b) 5X Plan APO Infinity Corrected Objective from another manufacturer showing 10% contrast at 330 lp/mm